Hands-on
workshop
”Real-time qPCR and HRM for genetic analysis”
TATAA
Biocenter qPCR Training course
This 2-day introductory
course consists of both theoretical and practical parts where you
get to perform Real-time qPCR experiments yourself under experienced
supervision. The course will give you a deeper understanding of the
fundamentals of qPCR, including primer and probe design, optimization of qPCR
reactions and basic data analysis. It will also introduce you to High
Resolution Melt analysis, HRM, which is a valuable method to analyze genetic
differences. DNA melting curves are used to distinguish differences of down to
single base substitutions and can be used for SNP analysis , Gene scanning or
Methylation analysis.
Date and Time:
10-11 June 2010, 09:00-17-00
Venue:
TATAA Biocenter, Odinsgatan 28, Gothenburg, Sweden. How to find.
For whom?:
Our highly appreciated
courses are suitable for researchers, technicians, post-graduate students or
anyone that wants to learn more about real-time PCR and real-time PCR related
technologies
Topics
Introduction
to PCR and qPCR theory
Detection
technologies
Basic data
analysis
Primer and
probe design
Optimization
of qPCR protocols
Quantification
strategies
Validation
of qPCR assays
Basics of
High Resolution Melt and common applications
Assay
design and optimization
Genotyping
Gene
scanning
Methylation
analysis
Registration
fee: University/Non profit: 620 Euro, Company: 760 Euro (Lunches and
refreshments are included)
TATAA Biocenter has given courses in the field of qPCR since 2001 and has over 50 publications in the area, including highlights in Nature journals, several patents and is supporting leading biopharma companies. We want to share our knowledge and experience also with you.
