including GenEx Light

For all gene expression studies using quantitative PCR it is necessary to compensate for differences between samples due to material losses, differences in RT yields and PCR inhibition. Normalization should include an endogenous control gene, but can also be complemented by identical sample input amounts. The endogenous control gene should have constant expression in all the samples compared. There is no universal control gene, expressed at a constant level under all conditions and in all tissues.

The best way to choose the proper reference gene is by running a panel of potential genes on a number of representative test samples. The gene(s) most appropriate for normalization are chosen in each case.

• Use your current supplier of mixes

• Experimentally validated on numerous instrument platforms

• Why design 12 assays when 10 are not interesting for you?

GenEx Light

Is new developed software based on a very user friendly windows platform. A complete data management system together with powerful tools like 2D and 3D graphics data visualization helps you editing pro processing of real-time PCR data.

Features for mail support, and viewlet live demonstrations makes it very easy to get started.

Live demonstration!

GenEX light includes both GeNorm and Normfinder to select optimum reference genes. There are cases where the GeNorm algorithm selects suboptimal reference genes and, likewise, there are cases where Normfinder is not appropriate. With GenEx Light you can always find the best gene for your system.

Materials included

• Primer sets for 12 candidate endogenous control genes.Assays for the most common endogenous control genes for gene expression studies, and provides a rapid and cost efficient way to identify your control genes for Human and Mouse respectively Enough for 100 rxns per gene.

• Positive Control DNA.

• The addition of real-time PCR regents is necessary. Primer sets are validated for use with most commercial available master mixes for qPCR containing SYBR Green I.

• GenEX Light

• Software including both GeNorm and Normfinder to select optimum reference genes.

Contacts and ordering

The Human Endogenous Control Panel Kit can be ordered from our distribution partners around the world.

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