Job openings12th December
Molecular microbiological identification and typing22nd November
Molecular microbiological identification and typing
TATAA Biocenter are proud to collaborate with RISE Research Institutes of Sweden and invites you to our course.
Course date: 17-18 Januari 2018. The courses start at 9 am and ends between 4.30 and 5.30 pm.
Registration deadline: 2 January 2018.
Course Language: Course will be held in English. Lecturers are Swedish.
- Day 1, RISE, Jordbruk och livsmedel, Frans Perssons väg 6, Göteborg
- Day 2, TATAA Biocenter, Odinsgatan 28, Göteborg
Responsible teachers: Charlotta Löfström PhD, Erik Nygren PhD, Johanna Edlund MSc, Filip Stern PhD
Number of students: Up to 15 students
Course fee: 2 days theoretical course inclusive laboratory experiment, 9000 SEK/attendee (excluded VAT). The course fee includes tuition, course materials, lunch, beverage, light snack, and fruits. Course fees DO NOT include accommodation, airfare or other travel costs.
Molecular analysis methods used for identification and typing of microorganisms are important to determine properties of the microbial strain, e.g. antimicrobial resistance, or to trace outbreaks in the food production chain. In recent years, novel technologies have been introduced based on thorough investigations of the nucleic acid content (DNA/RNA) of the microorganism.
This course provides you with an overview of some of these techniques and gives advice on how to interpret the complex data that are generated. The course focuses on quantitative PCR, whole genome sequencing and metagenomics. Some examples of software and approaches to data analysis of sequencing data will be provided.
Who should attend this course?
The target group for this course includes persons with experience with microbiological analysis methods in e.g. the food or pharmaceutical industry, who needs increased knowledge about methods used for microbial identification, with focus on new DNA/RNA based analysis techniques.
We recommend that you have good knowledge and experience with microbiological laboratory work.
After attending this course, participants will have obtained knowledge about molecular methods for identification and typing of microorganisms.
Focus in the course is on novel molecular techniques such as qPCR, whole genome sequencing and metagenomics. Some tools for data analysis will also be demonstrated.
End-of-year offer!16th November
Offer: Pick products from at least 2 of the workflow steps below:
RNA extraction solutions
- All RNA extraction kits from Norgen Biotek
- CelluLyser™ Lysis and cDNA Synthesis Kit
- CelluLyser™ Micro Lysis and cDNA Synthesis Kit
Quality control products
- Reference Gene Panels – find the most stable reference genes
- ValidPrime® – control for gDNA background
- TATAA Universal RNA and DNA Spikes – control for inhibition
- TATAA Interplate Calibrator – compensate for variations between qPCR runs
- Heat&Run gDNA Removal Kit
- PCR Decontamination Kit
- TATAA DeltaAmp Quality assay – a new molecular method to assess RNA/DNA integrity with qPCR
- VisiBlue™ – qPCR Mix Colorant
- BOXTO – control for Primer Dimers in Probe based qPCR assays
cDNA synthesis and qPCR reagents
- TATAA GrandScript cDNA Synthesis Kit
- TATAA GrandScript cDNA SuperMix
- TATAA GrandMaster and GenMaster mixes
*The 22% discount is valid for orders placed 16th of November to 20th of December 2017. Order directly from TATAA Biocenter in our webshop (webshop.tataa.com) or by e-mail to email@example.com, the discount will show in the order confirmation. The discount cannot be combined with other offers or discounts.
Expression profiling of circulating tumor cells13th October
Researchers from University of Essen in collaboration with Qiagen and TATAA stratify breast cancer patients based on expression profiling of circulating tumor cells
Circulating tumor cells (CTC) are thought to be an ideal surrogate marker to monitor disease progression in metastatic breast cancer (MBC).We investigated the prediction of treatment response in CTC of MBC patients on the basis of the expression of 46 genes.
CTCs are proposed to be an attractive source of liquid biopsy markers for monitoring disease progression. CTCs are highly heterogeneous and can change in number and phenotype under anticancer treatment. Stem cell-like tumor cells, undergoing epithelial-mesenchymal-transition (EMT) and not displaying the classical epithelial phenotype, are discussed as an active source of metastatic spread and have been identified within the population of CTCs.
Press release – Mic28th September
TATAA Biocenter Expands its real-time PCR offerings with the Mic from Bio Molecular Systems
Gothenburg. Sweden, and Upper Coomera, Australia, September 28, 2017
TATAA Biocenter, the world´s leading provider of hands-on training in molecular analyses, expands its qPCR instrument park with the Mic from Bio Molecular Systems. “People come from all around the world to TATAA for qPCR training and they expect getting chance to try out the best qPCR instruments there are. The Mic is certainly one of them and it will be most attractive option for those with space limitations. Also, with no moving parts, the Mic is essentially calibration free.” says Dr. Mikael Kubista, founder and CEO of TATAA Biocenter. TATAA Biocenter courses are arranged around the world supported by leading instrument and solution providers in molecular analyses. TATAA is also instrumental in quality control and standardization of molecular testing, being member of the European efforts SPIDIA (www.spidia.eu) and CANCER-ID (www.cancer-id.eu) standardizing the preanalytical steps and blood based analyses, respectively. “We are very proud of the collaboration with TATAA Biocenter. Now, users have the possibility to compare the Mic with traditional qPCR instruments and appreciate its advantages.”, says John Corbett, CEO of Bio Molecular Systems.
About TATAA Biocenter
TATAA Biocenter is world’s prime organizer of hands-on training in molecular diagnostics, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for quality control in nucleic acids testing. TATAA has the best equipped laboratories for qPCR based analysis in Europe, and provides full range of nucleic acids and protein analyses services using qPCR, dPCR, microarray and NGS to pharmaceutical and biotech companies in compliance with ISO 17025 standard. Founded in 2001, TATAA has facilities in Gothenburg, Sweden and Prague, Czech Republic. In 2013 TATAA was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.
About BIO Molecular Systems
Bio Molecular Systems (BMS) is a biotechnology company that designs, manufactures and distributes instrumentation for the life sciences. Established in 2010 and comprising of the founders and senior personnel from the former Corbett Life Sciences company, BMS has the expertise in developing innovative, versatile, robust, and easy-to-use products that generate strong customer loyalty. Our long-standing position in the biotech manufacturing sector enables us to establish a reputation for continuous and rapid in-house innovation and product evolution.
BMS has an R&D and manufacturing facility located within the Gold Coast area just 50 minutes south of the city of Brisbane (Queensland, Australia). Our facilities contain state of the art technology for both R&D engineering and manufacturing. We also have a second site in Sydney used to conduct all applications testing. By directly controlling all phases of production, we optimise quality control, minimise overhead costs and ensure the most rapid product development cycles.
For more information:
Comparison of WGA techniques for human single cell exome sequencing22nd September
Comparison of WGA techniques for human single cell exome sequencing
Whole genome amplification (WGA) is currently a prerequisite for single cell whole genome or exome sequencing. Depending on the method used the rate of artifact formation, allelic dropout and sequence coverage over the genome may differ significantly.
TATAA Biocenter is a proud distributor of the MALBAC kits and are pleased too see that MALBAC had the highest fidelity for both exome coverage and variant calling!
“The largest difference between the evaluated protocols was observed when analyzing the target coverage and read depth distribution. These differences also had impact on the downstream variant calling. Conclusively, the products from the AMPLI1 and MALBAC kits were shown to be most similar to the bulk samples and are therefore recommended for WGA of single cells.”
Comparison of whole genome amplification techniques for human single cell exome sequencing
Erik Borgström, Marta Paterlini, Jeff E. Mold, Jonas Frisen, Joakim Lundeberg
Published: February 16, 2017, https://doi.org/10.1371/journal.pone.0171566
Gothenburg. Sweden, and Jena, Germany, August 17, 2017
TATAA Biocenter Expands its real-time PCR offerings with Analytik Jena´s qTOWER3
TATAA Biocenter, the world´s leading provider of hands-on training in molecular analyses, expands its qPCR instrument park with Analytik Jena´s qTOWER3. “People come from all around the world to TATAA to be trained in qPCR, and they expect getting chance to try out the best qPCR instruments there are. The qTOWER3 is certainly one of them and it will become an attractive option to those looking for a new instrument.” says Dr. Mikael Kubista, founder and CEO of TATAA Biocenter. TATAA Biocenter courses are arranged around the world and supported by all the leading instrument and solution providers in molecular analyses. TATAA is also instrumental in quality control and standardization of molecular testing, being member of the European efforts SPIDIA (www.spidia.eu) and CANCER-ID (www.cancer-id.eu) standardizing the preanalytical steps and blood based analyses, respectively. “We are very proud of the collaboration with TATAA Biocenter. Now, we have the possibility to compare the qTOWER³ to similar qPCR instruments on the market. It is perfect for us to give users opportunity to test it head-to-head to other instruments on the market at the TATAA courses”, says Melanie Jahn, product manager at Analytik Jena.
About TATAA Biocenter
TATAA Biocenter is world’s largest organizer of hands-on training in molecular diagnostics, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for quality control in nucleic acids testing. TATAA has the best equipped laboratories for qPCR based analysis in Europe, and provides full range of nucleic acids and protein analyses services using qPCR, dPCR, microarray and NGS to pharmaceutical and biotech companies in compliance with ISO 17025 standard. Founded in 2001, TATAA has facilities in Gothenburg, Sweden and Prague, Czech Republic. In 2013 TATAA was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.
About Analytik Jena
Analytik Jena is a provider of instruments and products in the areas of analytical measuring technology and bioanalytical applications. The life science portfolio provides a wide spectrum for automated as well as individual solutions for molecular biology. The products are focused to offer quality and reproducibility of the laboratory results and encompasses powerful device systems and over 500 reagents and kits, including those for nucleic acid extraction, PCR, qPCR and pathogen analyses. The company’s in-house expertise is protected by more than 150 patents.
For more information see:
Olink Proteomics Webinar15th August
“Proteogenomic strategies to advance drug development and precision medicine”
TATAA Biocenter’s partner Olink Proteomics are sponsoring a Science/AAAS-hosted webinar: Gain new insights into how proteomics and genomics are now increasingly joining forces to improve the clinical utility of protein biomarkers and to enhance drug development strategies.
When? Wednesday September 6, 2017 at 12:00 pm U.S. Eastern Time/18:00 Central European Time
This free event will feature presentations from Professor Ulf Gyllensten (Uppsala University) & Dr. Anders Mälarstig (Pfizer Sweden)
During the webinar, the speakers will:
- Discuss how covariates (genetic, environmental, and clinical) can affect the levels of potential biomarkers
- Explore the use of covariate modeling to establish personally normalized plasma protein profiles (PNPPP) to reduce non-disease-related variation and maximize clinical potential
- Describe how protein quantitative trait loci (pQTLs) can provide insights into disease biology and improve the efficiency of drug development
- Answer your questions live during the broadcast!
Press release – Two-tailed PCR for microRNA13th July
TATAA Biocenter and BTU develops Two-tailed PCR for microRNA analysis offering superior sensitivity and specificity
TATAA Biocenter and the Institute of Biotechnology, BIOCEV, has developed Two-Tailed PCR for ultrasensitive analysis of microRNAs based on products from Roche. Based on an innovative novel design with a RT primer sensing the microRNA using two connected hemi-probes exceeding sensitivity and superior specificity is achieved. The results of the work appeared in this week’s issue of the prestigious journal Nucleic Acids Research.
Mature microRNAs are single-stranded molecules only 22-24 nucleotides in length. They constitute a recently discovered class of non-coding RNAs that plays a key role in the regulation of gene expression. Many microRNAs are present in families that differ only in a single base position or terminal modification. Acting at the post-transcriptional level, microRNAs fine-tune the expression of many protein-encoding genes. microRNAs are present in tissues, but also in biofluids, where they are actively transported as reporter molecules. This feature makes microRNAs exceedingly attractive biomarkers and its use is currently being standardized by the European effort SPIDIA.
There are several techniques to measure microRNA levels with quantitative PCR, but current methods use conventional pairs of primers that each is 20-25 bases in lengths. To be able to amplify the short microRNAs they must be elongated, which often compromises sensitivity and specificity. The novel Two-tailed approach solves this problem by sensing the microRNA sequencing with two short hemiprobes. Neither hemiprobe is long enough to bind alone, but in the novel Two-Tailed design the hemiprobes are connected, which leads to cooperative binding, such that either both bind, which gives sufficient contact and stability, or neither binds. Since each hemiprobe is short, a mismatch in its binding site has major impact, leading to the enhanced specificity of the Two-tailed method.
Two-tailed PCR was compared to three other methods for microRNA detection and found to be more sensitive and specific, being capable to detect fewer than 10 microRNA molecules in a sample. Two-tailed PCR can be multiplexed to essentially any degree using 2-tube protocol and when using one-tube protocol multiplexing with probes is possible. Two-tailed PCR is one of the methods currently evaluated for microRNA detection in liquid biopsies collected for the management of cancer within the European effort Cancer-ID and for Sepsis within the European effort Smartdiagnos. Custom Two-tailed assays can be ordered.
About TATAA Biocenter
TATAA Biocenter is world’s largest organizer of hands-on training in quantitative real-time PCR and Next Generation Sequencing, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for nucleic acids analysis. TATAA Biocenter offers a seamless workflow for high quality nucleic acids analysis from experimental design, sample preparation, quality control, and analysis, meeting the specific requirements for compliance with ISO17025 standard. Founded in 2001, TATAA Biocenter has laboratories in Gothenburg, Sweden, and in Prague, Czech Republic. In 2013 TATAA Biocenter was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.
For more information see:
or contact us on firstname.lastname@example.org
Summer offers – GenMaster mix and GrandPerformance assays28th June
- Are you running a large study?
- Do you want a qPCR mastermix delivering reliable results?
- Do you want to avoid protocol optimization?
TATAA Biocenter are proud to present our new TATAA GenMaster Mixes and would like to give you a chance to try it out for an even more affordable price! During July and August you can order all sizes for the cost of an ice-cream (almost).
- Wet-lab validated probe assays
- Preamp validated to not introduce bias
- Probe assays included for free in qPCR profiling service
Highly sensitive and extensively optimized qPCR assays
TATAA GrandPerformance assays are the only assays on the market validated for single cell profiling. GrandPerformance Cancer Assays can be mixed in any combination and used in a preamplification workflow offering a sensitivity down to a few transcripts under optimum conditions. Profile individual circulating tumor cells from patients revealing heterogeneity:
*The 19% discount is valid July to August 2017 on the products in Ordering Information. Order directly from TATAA Biocenter in our webshop or by e-mail to email@example.com. The discount cannot be combined with other offers or discounts.
**The 50% discount is valid July to August 2017 on the TATAA GrandPerformance assays only. Order directly from TATAA Biocenter by e-mail to firstname.lastname@example.org. The discount cannot be combined with other offers or discounts.