Expression profiling of circulating tumor cells

13th October

Researchers from University of Essen in collaboration with Qiagen and TATAA stratify breast cancer patients based on expression profiling of circulating tumor cells

Circulating tumor cells (CTC) are thought to be an ideal surrogate marker to monitor disease progression in metastatic breast cancer (MBC).We investigated the prediction of treatment response in CTC of MBC patients on the basis of the expression of 46 genes.

CTCs are proposed to be an attractive source of liquid biopsy markers for monitoring disease progression. CTCs are highly heterogeneous and can change in number and phenotype under anticancer treatment. Stem cell-like tumor cells, undergoing epithelial-mesenchymal-transition (EMT) and not displaying the classical epithelial phenotype, are discussed as an active source of metastatic spread and have been identified within the population of CTCs.

> Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to Therapy in Metastatic Breast Cancer-Clin Chem (2017)

TATAA GrandPerformance Assays – Cancer Panel

AdnaTest platform

TATAA PreAmp GrandMaster® Mix

ValidPrime® Control for Genomic Background

 

Press release – Mic

28th September

TATAA Biocenter Expands its real-time PCR offerings with the Mic from Bio Molecular Systems

Gothenburg. Sweden, and Upper Coomera, Australia, September 28, 2017

TATAA Biocenter, the world´s leading provider of hands-on training in molecular analyses, expands its qPCR instrument park with the Mic from Bio Molecular Systems. “People come from all around the world to TATAA for qPCR training and they expect getting chance to try out the best qPCR instruments there are. The Mic is certainly one of them and it will be most attractive option for those with space limitations. Also, with no moving parts, the Mic is essentially calibration free.” says Dr. Mikael Kubista, founder and CEO of TATAA Biocenter. TATAA Biocenter courses are arranged around the world supported by leading instrument and solution providers in molecular analyses. TATAA is also instrumental in quality control and standardization of molecular testing, being member of the European efforts SPIDIA (www.spidia.eu) and CANCER-ID (www.cancer-id.eu) standardizing the preanalytical steps and blood based analyses, respectively. “We are very proud of the collaboration with TATAA Biocenter. Now, users have the possibility to compare the Mic with traditional qPCR instruments and appreciate its advantages.”, says John Corbett, CEO of Bio Molecular Systems.

About TATAA Biocenter

TATAA Biocenter is world’s prime organizer of hands-on training in molecular diagnostics, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for quality control in nucleic acids testing. TATAA has the best equipped laboratories for qPCR based analysis in Europe, and provides full range of nucleic acids and protein analyses services using qPCR, dPCR, microarray and NGS to pharmaceutical and biotech companies in compliance with ISO 17025 standard. Founded in 2001, TATAA has facilities in Gothenburg, Sweden and Prague, Czech Republic. In 2013 TATAA was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.

About BIO Molecular Systems

Bio Molecular Systems (BMS) is a biotechnology company that designs, manufactures and distributes instrumentation for the life sciences. Established in 2010 and comprising of the founders and senior personnel from the former Corbett Life Sciences company, BMS has the expertise in developing innovative, versatile, robust, and easy-to-use products that generate strong customer loyalty. Our long-standing position in the biotech manufacturing sector enables us to establish a reputation for continuous and rapid in-house innovation and product evolution.
BMS has an R&D and manufacturing facility located within the Gold Coast area just 50 minutes south of the city of Brisbane (Queensland, Australia). Our facilities contain state of the art technology for both R&D engineering and manufacturing. We also have a second site in Sydney used to conduct all applications testing. By directly controlling all phases of production, we optimise quality control, minimise overhead costs and ensure the most rapid product development cycles.

For more information:

Contact us on info@tataa.com
TATAA Biocenter hands-on training program
www.mic-pcr.com
www.biomolecularsystems.com

Comparison of WGA techniques for human single cell exome sequencing

22nd September

Comparison of WGA techniques for human single cell exome sequencing

Whole genome amplification (WGA) is currently a prerequisite for single cell whole genome or exome sequencing. Depending on the method used the rate of artifact formation, allelic dropout and sequence coverage over the genome may differ significantly.

TATAA Biocenter is a proud distributor of the MALBAC kits and are pleased too see that MALBAC had the highest fidelity for both exome coverage and variant calling!

“The largest difference between the evaluated protocols was observed when analyzing the target coverage and read depth distribution. These differences also had impact on the downstream variant calling. Conclusively, the products from the AMPLI1 and MALBAC kits were shown to be most similar to the bulk samples and are therefore recommended for WGA of single cells.”


> MALBAC Single Cell WGA Kit

Comparison of whole genome amplification techniques for human single cell exome sequencing
Erik Borgström, Marta Paterlini, Jeff E. Mold, Jonas Frisen, Joakim Lundeberg 
Published: February 16, 2017, https://doi.org/10.1371/journal.pone.0171566

 

17th August

Gothenburg. Sweden, and Jena, Germany, August 17, 2017

TATAA Biocenter Expands its real-time PCR offerings with Analytik Jena´s qTOWER3

TATAA Biocenter, the world´s leading provider of hands-on training in molecular analyses, expands its qPCR instrument park with Analytik Jena´s qTOWER3. “People come from all around the world to TATAA to be trained in qPCR, and they expect getting chance to try out the best qPCR instruments there are. The qTOWER3 is certainly one of them and it will become an attractive option to those looking for a new instrument.” says Dr. Mikael Kubista, founder and CEO of TATAA Biocenter. TATAA Biocenter courses are arranged around the world and supported by all the leading instrument and solution providers in molecular analyses. TATAA is also instrumental in quality control and standardization of molecular testing, being member of the European efforts SPIDIA (www.spidia.eu) and CANCER-ID (www.cancer-id.eu) standardizing the preanalytical steps and blood based analyses, respectively. “We are very proud of the collaboration with TATAA Biocenter. Now, we have the possibility to compare the qTOWER³ to similar qPCR instruments on the market. It is perfect for us to give users opportunity to test it head-to-head to other instruments on the market at the TATAA courses”, says Melanie Jahn, product manager at Analytik Jena.

About TATAA Biocenter

TATAA Biocenter is world’s largest organizer of hands-on training in molecular diagnostics, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for quality control in nucleic acids testing. TATAA has the best equipped laboratories for qPCR based analysis in Europe, and provides full range of nucleic acids and protein analyses services using qPCR, dPCR, microarray and NGS to pharmaceutical and biotech companies in compliance with ISO 17025 standard. Founded in 2001, TATAA has facilities in Gothenburg, Sweden and Prague, Czech Republic. In 2013 TATAA was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.

About Analytik Jena

Analytik Jena is a provider of instruments and products in the areas of analytical measuring technology and bioanalytical applications. The life science portfolio provides a wide spectrum for automated as well as individual solutions for molecular biology. The products are focused to offer quality and reproducibility of the laboratory results and encompasses powerful device systems and over 500 reagents and kits, including those for nucleic acid extraction, PCR, qPCR and pathogen analyses. The company’s in-house expertise is protected by more than 150 patents.

For more information see:

> Visit us at www.tataa.com or contact us on info@tataa.com

TATAA Biocenter hands-on training program

Analytik Jena 

The qTOWER3

Olink Proteomics Webinar

15th August

“Proteogenomic strategies to advance drug development and precision medicine”

TATAA Biocenter’s partner Olink Proteomics are sponsoring a Science/AAAS-hosted webinar:  Gain new insights into how proteomics and genomics are now increasingly joining forces to improve the  clinical utility of protein biomarkers and to enhance drug development strategies.

When? Wednesday September 6, 2017 at 12:00 pm U.S. Eastern Time/18:00 Central European Time

This free event will feature presentations from Professor Ulf Gyllensten (Uppsala University) & Dr. Anders Mälarstig (Pfizer Sweden)

During the webinar, the speakers will:

  • Discuss how covariates (genetic, environmental, and clinical) can affect the levels of potential biomarkers
  • Explore the use of covariate modeling to establish personally normalized plasma protein profiles (PNPPP) to reduce non-disease-related variation and maximize clinical potential
  • Describe how protein quantitative trait loci (pQTLs) can provide insights into disease biology and improve the efficiency of drug development
  • Answer your questions live during the broadcast!

> Read more about the webinar here

> Register directly here

Press release – Two-tailed PCR for microRNA

13th July

TATAA Biocenter and BTU develops Two-tailed PCR for microRNA analysis offering superior sensitivity and specificity

TATAA Biocenter and the Institute of Biotechnology, BIOCEV, has developed Two-Tailed PCR for ultrasensitive analysis of microRNAs based on products from Roche. Based on an innovative novel design with a RT primer sensing the microRNA using two connected hemi-probes exceeding sensitivity and superior specificity is achieved. The results of the work appeared in this week’s issue of the prestigious journal Nucleic Acids Research.

Mature microRNAs are single-stranded molecules only 22-24 nucleotides in length. They constitute a recently discovered class of non-coding RNAs that plays a key role in the regulation of gene expression. Many microRNAs are present in families that differ only in a single base position or terminal modification. Acting at the post-transcriptional level, microRNAs fine-tune the expression of many protein-encoding genes. microRNAs are present in tissues, but also in biofluids, where they are actively transported as reporter molecules. This feature makes microRNAs exceedingly attractive biomarkers and its use is currently being standardized by the European effort SPIDIA.

There are several techniques to measure microRNA levels with quantitative PCR, but current methods use conventional pairs of primers that each is 20-25 bases in lengths. To be able to amplify the short microRNAs they must be elongated, which often compromises sensitivity and specificity. The novel Two-tailed approach solves this problem by sensing the microRNA sequencing with two short hemiprobes. Neither hemiprobe is long enough to bind alone, but in the novel Two-Tailed design the hemiprobes are connected, which leads to cooperative binding, such that either both bind, which gives sufficient contact and stability, or neither binds. Since each hemiprobe is short, a mismatch in its binding site has major impact, leading to the enhanced specificity of the Two-tailed method.

Two-tailed PCR was compared to three other methods for microRNA detection and found to be more sensitive and specific, being capable to detect fewer than 10 microRNA molecules in a sample. Two-tailed PCR can be multiplexed to essentially any degree using 2-tube protocol and when using one-tube protocol multiplexing with probes is possible. Two-tailed PCR is one of the methods currently evaluated for microRNA detection in liquid biopsies collected for the management of cancer within the European effort Cancer-ID and for Sepsis within the European effort Smartdiagnos. Custom Two-tailed assays can be ordered.

About TATAA Biocenter

TATAA Biocenter is world’s largest organizer of hands-on training in quantitative real-time PCR and Next Generation Sequencing, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for nucleic acids analysis. TATAA Biocenter offers a seamless workflow for high quality nucleic acids analysis from experimental design, sample preparation, quality control, and analysis, meeting the specific requirements for compliance with ISO17025 standard. Founded in 2001, TATAA Biocenter has laboratories in Gothenburg, Sweden, and in Prague, Czech Republic. In 2013 TATAA Biocenter was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.

For more information see:

BTU

BIOCEV

NUCLEIC ACIDS REASEARCH

SPIDIA

CANCER-ID

SMARTDIAGNOS

TATAA miRNA 2-Tailed RT-qPCR assays

or contact us on info@tataa.com

Summer offers – GenMaster mix and GrandPerformance assays

28th June

→ Sun, beach, ice-cream….. and 19% discount* on GenMaster!

  • Are you running a large study?
  • Do you want a qPCR mastermix delivering reliable results?
  • Do you want to avoid protocol optimization?

TATAA Biocenter are proud to present our new TATAA GenMaster Mixes and would like to give you a chance to try it out for an even more affordable price! During July and August you can order all sizes for the cost of an ice-cream (almost).


→ 50 % summer discount!** on TATAA GrandPerformance Cancer Assays

  • Wet-lab validated probe assays
  • Preamp validated to not introduce bias
  • Probe assays included for free in qPCR profiling service

Highly sensitive and extensively optimized qPCR assays

TATAA GrandPerformance assays are the only assays on the market validated for single cell profiling. GrandPerformance Cancer Assays can be mixed in any combination and used in a preamplification workflow offering a sensitivity down to a few transcripts under optimum conditions. Profile individual circulating tumor cells from patients revealing heterogeneity:


*The 19% discount is valid July to August 2017 on the products in Ordering Information. Order directly from TATAA Biocenter in our webshop or by e-mail to order@tataa.com. The discount cannot be combined with other offers or discounts.

**The 50% discount is valid July to August 2017 on the TATAA GrandPerformance assays only. Order directly from TATAA Biocenter by e-mail to order@tataa.com. The discount cannot be combined with other offers or discounts.

Midsummer Offer – GrandMaster

22nd June

Do you know where you have your primers?

Midsummer Offer – Buy GrandMaster and receive assay design and validation for 150 €!*

Original MIQE guidelines required primer disclosure, because the exact location of primers in a PCR reaction is critical. If a primer targets a sequence with variation the assay may not perform in certain samples. Many of the assays deposited in databases, published in literature and even offered commercially where developed a long time ago, when information about sequence variation was scarce.

So, even if the assays where designed according to best principles they may not perform to expectations. To check the design using updated sequence information requires knowing the primer sequence. However, most commercial assays do not disclose those and provide only a context sequence in compliance with the primer sequence disclosure added to the MIQE guidelines to satisfy industry requirements.

Further, most assays were designed for use on classical samples, where the amount of material is plenty. They perform well on those samples, but are frequently not robust and specific enough for use on more challenging samples, such as single cells and multiplex assays.

Therefore, at TATAA, we always design and validate our own assays and assays for our customers. Our validations are stringent and include estimating PCR efficiency including confidence interval, dynamic range, linearity, repeatability and Limit of Quantification. For diagnostic assays, we also include Limit of Detection including confidence interval and, when necessary, cross reactivity and robustness. Of course, all assays are validated to produce a single product and that side products such as primer dimers are negligible. One example of validation is available on Labroot.

We want you to be successful too!

In Sweden Midsummer is a major holiday, with dancing like frogs around an up-side down ancient symbol.

At TATAA we celebrate Midsummer by offering all customers that order a TATAA GrandMaster mix assay design and validation from only 150 €* through our partner!

Place your order today and you will receive an order form for the design!

*Midsummer offer valid until July 30, 2017. Design includes validation based on standard curve estimating PCR efficiency incl., confidence interval, dynamic range, R2, and LoQ. Melt curve analysis is performed to validate a single product. NTC is run to validate negligible primer dimer formation. The design and validation is done by our partner GeneCore at BIOCEV.

Expression profiling of single circulating tumor cells

16th June

TATAA Biocenter launches GrandPerformance assays for expression profiling of single circulating tumor cells

TATAA Biocenter, Europe´s leading provider of genomic services using quantitative real-time PCR (qPCR), launches the GrandPerformance qPCR assays for the profiling of Circulating Tumor Cells. The assays are optimized for single cell sensitivity and the workflow is based on preamplification allowing sensitive and precise measurements of 96 specific expression markers per cell.

Tumors may shed cells into the blood flow that evade the immune defence and may seed metastases. These cells are called circulating tumor cells (CTCs). CTCs may be detected in blood sample and their presence is a negative prognostic indicator. Further characterization of CTCs by expression profiling may reveal their phenotype, which may guide therapeutic decisions. Molecular characterization of CTCs is challenging, because of their very low abundance, which requires exceedingly sensitive and specific methods. TATAA Biocenter has previously developed workflow for exceedingly specific and sensitive single cell expression profiling based on reverse transcription (RT), preamplification and real-time PCR (Expert review of molecular diagnostics 14, 323 (2014)). Now TATAA launches a panel of over 100 GrandPerformance assays wet-lab validated for CTC profiling that allows the characterization of individual CTCs. The panel was recently used by the group of professor Klaus Pantel at University Klinik Hamburg-Eppendorf to profile individual CTCs collected from patient samples revealing heterogeneity among them (Clinical Chemistry 62 (11), 1504-1515 (2016)).


About TATAA Biocenter

TATAA Biocenter is the world’s largest organizer of hands-on training in quantitative real-time PCR and Next Generation Sequencing, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for nucleic acids analysis. TATAA Biocenter offers a seamless workflow for high quality nucleic acids analysis from experimental design, sample preparation, quality control, and analysis, meeting the specific requirements for compliance with ISO17025 standard. Founded in 2001, TATAA Biocenter has laboratories in Gothenburg, Sweden, and in Prague, Czech Republic. In 2013 TATAA Biocenter was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.


For more information on the TATAA GrandPerformance assays, see https://webshop.tataa.com/category.html/qpcr-assays or contact us on info@tataa.com

Lexogen Research Award

10th May

Supplier_Lexogen

Sequencing for all! – An award for difficult RNA samples

“High quality from low quality: Accurate gene expression profiling of low quality or FFPE samples“

Gene expression profiling using Next Generation Sequencing (NGS) is empowering an ever-increasing range of researchers to answer highly relevant biological and medical questions. Lexogen are dedicated to developing innovative technologies for NGS and making these accessible for all scientists.

With the Lexogen Research Award Lexogen wish to provide a chance for researchers to utilize more NGS. Submit a description of the project where you would use NGS and Lexogen’s RNA-Seq sample prep.

The winners shall be given a product of Lexogen together with a free sequencing run and data analysis.

» Read more and apply

» Products from Lexogen