Midsummer Offer – GrandMaster22nd June
Do you know where you have your primers?
Midsummer Offer – Buy GrandMaster and receive assay design and validation for 150 €!*
Original MIQE guidelines required primer disclosure, because the exact location of primers in a PCR reaction is critical. If a primer targets a sequence with variation the assay may not perform in certain samples. Many of the assays deposited in databases, published in literature and even offered commercially where developed a long time ago, when information about sequence variation was scarce.
So, even if the assays where designed according to best principles they may not perform to expectations. To check the design using updated sequence information requires knowing the primer sequence. However, most commercial assays do not disclose those and provide only a context sequence in compliance with the primer sequence disclosure added to the MIQE guidelines to satisfy industry requirements.
Further, most assays were designed for use on classical samples, where the amount of material is plenty. They perform well on those samples, but are frequently not robust and specific enough for use on more challenging samples, such as single cells and multiplex assays.
Therefore, at TATAA, we always design and validate our own assays and assays for our customers. Our validations are stringent and include estimating PCR efficiency including confidence interval, dynamic range, linearity, repeatability and Limit of Quantification. For diagnostic assays, we also include Limit of Detection including confidence interval and, when necessary, cross reactivity and robustness. Of course, all assays are validated to produce a single product and that side products such as primer dimers are negligible. One example of validation is available on Labroot.
We want you to be successful too!
In Sweden Midsummer is a major holiday, with dancing like frogs around an up-side down ancient symbol.
At TATAA we celebrate Midsummer by offering all customers that order a TATAA GrandMaster mix assay design and validation from only 150 €* through our partner!
Place your order today and you will receive an order form for the design!
*Midsummer offer valid until July 30, 2017. Design includes validation based on standard curve estimating PCR efficiency incl., confidence interval, dynamic range, R2, and LoQ. Melt curve analysis is performed to validate a single product. NTC is run to validate negligible primer dimer formation. The design and validation is done by our partner GeneCore at BIOCEV.
Expression profiling of single circulating tumor cells16th June
TATAA Biocenter launches GrandPerformance assays for expression profiling of single circulating tumor cells
TATAA Biocenter, Europe´s leading provider of genomic services using quantitative real-time PCR (qPCR), launches the GrandPerformance qPCR assays for the profiling of Circulating Tumor Cells. The assays are optimized for single cell sensitivity and the workflow is based on preamplification allowing sensitive and precise measurements of 96 specific expression markers per cell.
Tumors may shed cells into the blood flow that evade the immune defence and may seed metastases. These cells are called circulating tumor cells (CTCs). CTCs may be detected in blood sample and their presence is a negative prognostic indicator. Further characterization of CTCs by expression profiling may reveal their phenotype, which may guide therapeutic decisions. Molecular characterization of CTCs is challenging, because of their very low abundance, which requires exceedingly sensitive and specific methods. TATAA Biocenter has previously developed workflow for exceedingly specific and sensitive single cell expression profiling based on reverse transcription (RT), preamplification and real-time PCR (Expert review of molecular diagnostics 14, 323 (2014)). Now TATAA launches a panel of over 100 GrandPerformance assays wet-lab validated for CTC profiling that allows the characterization of individual CTCs. The panel was recently used by the group of professor Klaus Pantel at University Klinik Hamburg-Eppendorf to profile individual CTCs collected from patient samples revealing heterogeneity among them (Clinical Chemistry 62 (11), 1504-1515 (2016)).
About TATAA Biocenter
TATAA Biocenter is the world’s largest organizer of hands-on training in quantitative real-time PCR and Next Generation Sequencing, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for nucleic acids analysis. TATAA Biocenter offers a seamless workflow for high quality nucleic acids analysis from experimental design, sample preparation, quality control, and analysis, meeting the specific requirements for compliance with ISO17025 standard. Founded in 2001, TATAA Biocenter has laboratories in Gothenburg, Sweden, and in Prague, Czech Republic. In 2013 TATAA Biocenter was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.
For more information on the TATAA GrandPerformance assays, see https://webshop.tataa.com/category.html/qpcr-assays or contact us on email@example.com
Lexogen Research Award10th May
Sequencing for all! – An award for difficult RNA samples
“High quality from low quality: Accurate gene expression profiling of low quality or FFPE samples“
Gene expression profiling using Next Generation Sequencing (NGS) is empowering an ever-increasing range of researchers to answer highly relevant biological and medical questions. Lexogen are dedicated to developing innovative technologies for NGS and making these accessible for all scientists.
With the Lexogen Research Award Lexogen wish to provide a chance for researchers to utilize more NGS. Submit a description of the project where you would use NGS and Lexogen’s RNA-Seq sample prep.
The winners shall be given a product of Lexogen together with a free sequencing run and data analysis.
First impressions from a great symposium!5th May
First impressions from a great symposium!
8th Gene Quantification Event — qPCR dPCR & NGS 2017
Psst – Don’t miss CEO Mikael Kubista and Commissioned Services manager Jens Björkman 🙂
Free one day Symposium Gothenburg25th April
Join us for an exciting one-day symposium, “Protein biomarker discovery in clinical & epidemiological research“. The event is being jointly arranged by the TATAA Biocenter in Göteborg and Olink Proteomics.
TATAA Biocenter växer och söker nya medarbetare!29th March
TATAA Biocenter växer och söker nya medarbetare!
Är du intresserad av att bli del av ett spännande team, läs mer>>
New Webshop23rd February
New Webshop – We Are Proud to Present our New Webshop!
webshop.tataa.com or click WEBSHOP in the top menu bar
Recruitments at TATAA Biocenter3rd February
TATAA Biocenter söker en erfaren Säljare.
Merry Christmas and a Happy New Year15th December
TATAA Biocenter wish you all a
qPCR & NGS Courses Spring 2017 – Registration is open!21st November
qPCR & NGS Courses Spring 2017 – Registration is open!
Visit Gothenburg or Prague