Comparison of WGA techniques for human single cell exome sequencing22nd September
Comparison of WGA techniques for human single cell exome sequencing
Whole genome amplification (WGA) is currently a prerequisite for single cell whole genome or exome sequencing. Depending on the method used the rate of artifact formation, allelic dropout and sequence coverage over the genome may differ significantly.
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“The largest difference between the evaluated protocols was observed when analyzing the target coverage and read depth distribution. These differences also had impact on the downstream variant calling. Conclusively, the products from the AMPLI1 and MALBAC kits were shown to be most similar to the bulk samples and are therefore recommended for WGA of single cells.”
Comparison of whole genome amplification techniques for human single cell exome sequencing
Erik Borgström, Marta Paterlini, Jeff E. Mold, Jonas Frisen, Joakim Lundeberg
Published: February 16, 2017, https://doi.org/10.1371/journal.pone.0171566