Quantity and quality testing of RNA, miRNA, DNA, and protein
At TATAA Genomics Core Facility you have access to many methods and instruments for quantifying the amounts of biomolecules and testing samples for quality, integrity and purity. This allows you to ascertain highest quality and reproducibility of your protocols, which is essential for any downstream processing and analyses. We have systems that require minute sampe volumes (less than 2 µl), making sure you don’t lose more of your sample than necessary for further analyses. Our platforms for automated gel electrophoresis make your analyses fast, simple and highly cost efficient.
IN OUR FACILITY IN GÖTEBORG:
Sample Preparation lab
Our facility at TATAA Biocenter Göteborg is divided into separate labs to prevent contamination. Nucleic acids are extracted in the Sample Preparation lab, and also tested for quality and quantity. When there is a risk for hazardous fumes the Air Curtain Cabinet can be used. This lab contains several systems for nucleic acid extractions as well as for quality and quantity testing. General lab equipment include two heating blocks, centrifuges and a vortex.
Instruments – Quantity and quality testing of RNA, miRNA, DNA, and protein
Advanced Analytical Fragment Analyzer™ Automated CE System
The Fragment Analyzer™ Automated CE System is a fluorescence-based capillary electrophoresis instrument for quantification and qualification of NGS fragments, quantification and qualification of RNA and genomic DNA and Mutation Detection, also known as TILLiNG. By using a sensitive intercalating dye coupled with a LED light source, Fragment Analyzer™ Automated CE System obviates the need for fluorescent labeled primers. 12 samples can be analyzed in one run and up to 3 x 96 well plates can be placed on the instrument at one time, allowing up to 288 samples to be analyzed with minimal hands-on time. The Fragment Analyzer™ Automated CE System measures RQN (RNA Quality Number). Derived from PROSize® analytical software, RQN assesses the quality or integrity of total RNA. The system has the greatest sensitivity, the highest separation resolution over a wide range, the fastest separation times and widest dynamic range on the market (<40,000bp fragments).
Agilent 2100 BioAnalyzer
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Up to 12 samples can be run simultaneously and 2 µl of the sample is enough to load to each well on the chip. In 30-40 minutes the run is ready and you can analyze your obtained data. With the RIN algorithm (RNA Integrity Number) you get a number of the quality of your RNA sample.
Agilent 2200 TapeStation
Agilent 2200 TapeStation analyzes RNA and DNA without any gel preparation or chip priming. 1-96 samples can be loaded, separated, imaged and analyzed in only one minute per sample (batch prepare all 96 samples and run them in 2 hours). This automated platform calculates the RINe-value (equivalent to RIN) through mathematical analysis of the RNA profile, allowing objective quality scoring of eucaryotic total RNA.
NanoDrop ND-1000 Spectrophotometer
The NanoDropTM 1000 Spectrophotometer enables highly accurate UV/Vis analyses of samples with remarkable reproducibility. The patented sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions. The ND-1000 is an excellent instrument to measure the concentration of DNA, RNA and protein. To some degree, purity from phenol and proteins in RNA samples can also be evaluated. The NanoDrop uses only about 1-2 µl of the sample and there is no risk of contamination of the original sample.
NanoDrop ND-3300 Fluorospectrometer
NanoDrop ND-3300 makes a broad spectrum fluorescence analysis quick and easy. It uses fluorescence emission (400-750 nm) for measuring fluorophores and reagents in samples as small as 1 µl. UV, Blue and White LED excitation covers a broad wavelength range and makes it possible to analyze multiple emission profiles from a single sample.
Sigma 1-14 centrifuge – for spin columns
This high speed small-footprint microlitre centrifuge is suited for centrifuging 24x 1.5/2 ml tubes, great to use during extraction with spin columns. The acceleration and deceleration times are short. The maximum spin is 14 800 rpm/16 162 x g. It is easy to handle with big buttons and bright LCD display.
The Trinean DropSense96 is a multichannel spectrophotometer for quick and precise UV/VIS spectral analysis of microliter droplets of DNA/RNA, protein or small compounds. This spectrophotometer has all the characteristics of a plate reader, but uses specific DropPlates with 1-3 µl samples.
The key features for the Dropsense96 include full UV/VIS spectral analysis, specific quantification of biomolecules (e.g. DNA, RNA) in the presence of contaminants (cDrop), fast measurements < 5 minutes to read a full 96well plate, can be integrated into a liquid handling robot, etc.