Quality control in molecular diagnostics

Learn how to validate and control your qPCR assays for proper use in Molecular diagnostic settings.

Testimonial from previous course participant:

“New things to learn, very practical and clear” Verenice Ramirez Rodriguez, Roche Life Sciences, Mexico



1 DAY Quality control in molecular diagnostics

Target audience: Beginners to Advanced qPCR users

Entrance qualifications: Hands-on qPCR module or similar knowledge

Description: This course will give you the tools in how to do proper quality control of your qPCR assays to be used in molecular diagnostics. It will describe which controls that is needed, how they should be performed and the statistics on how to do the evaluations.

The course contains:

Introduction to statistics for qPCR

  • Principles of statistics.
  • Which type of statistics is suitable for qPCR?
  • Gaussian distribution, mean, standard deviation, confidence intervals.

Absolute quantification and standard curves

  • What is a standard curve?
  • What type of standard material should be used?
  • What does linear regression mean?
  • How are the calculations performed?

qPCR assay validation

  • How to validate a qPCR assay, which factors are important?
  • How to calculate Limit of Detection.
  • How to calculate Limit of Quantification.

Accuracy and precision

  • How is accuracy tested?
  • How is precision tested?
  • Reference materials and standards.
  • Which controls should be included?

Hands-on lab analyzing efficiency, repeatability, LOD and LOQ for a qPCR assay. Quantity of unknown samples are determined by standard curve analysis. Demonstration of how the software GenEx easily can be used for calculations of the parameters.