Life Science Sweden embraces ValidPrime™20th May
Life Science Sweden embraces ValidPrime™
- Offers a cost-efficient alternative to RT(-) controls
- Accurately validates qPCR assays in terms of their sensitivity to gDNA
- Allows correction for gDNA-derived signals
- Reduces the need for DNase treatment
Figure 1: Total number of RT and qPCR controls needed to check for gDNA background using traditional RT(-) approach and ValidPrime™. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT(-) reactions plus m x n qPCR controls, while using ValidPrime™ only m + n + 1 controls are needed.