CelluLyser Lysis and cDNA Synthesis Kit
Rapid and sensitive lysis and reverse transcription of small cell samples
The optimized CelluLyser buffer and powerful reverse transcription reagents maximize the yield of cDNA, making CelluLyser ideal for studies of small samples intended for real-time PCR.
Description
The CelluLyser Lysis and cDNA Synthesis kit enables you to analyze small samples from 10 000 cells down to a single cell with minimal losses and hands-on time. The CelluLyser kit can be used with collected cells (FACS sorted, patch clamp collected cells etc.), as well as cultured cells (both adherent and in solution), and has successfully been tested on a number of different mammalian cell lines.
Figure 1. Workflow for CelluLyser Lysis and cDNA Synthesis Kit.
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> Robust analysis of small samples - as little as 1 cell |
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> 100 % use of RNA content |
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> Simplicity - minimal hands-on time |
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> Avoid sample loss - one tube format |
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> Rapid lysis in only 10 minutes |
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> No expensive purification equipment needed |
Figure 2. Robust analysis of fractions of single cells. THP-1 single cells were sorted into separate tubes using FACS, cDNA was prepared using the CelluLyser Kit. Expression of b-actin was measured in 1/10th of a cell using real-time qPCR and gives insight into the heterogenity of a cell population.
Figure 3. Significant reduction of hands-on time and sample loss. Compared to standard column based purification methods, CelluLyser saves a considerable amount of time and material. 10 000 cells were used for analysis.
Content
Depending on cell amount and vessel format:
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CelluLyser Buffer |
250 or 100 reactions |
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cDNA Synthesis Reagents |
100 or 40 reactions |
The CelluLyser Buffer can be purchased separately, contact order@tataa.com for more information or order it here in the webshop.
Manual and product flyer
To download the manual or product flyer please visit Product Downloads.
Contacts and ordering
Place your order here in the webshop or order it by e-mailing order@tataa.com
Shipping costs will apply, please visit General terms and Shipping for information.
