Control for genomic background

ValidPrime® replaces the need to perform no reverse transcriptase (RT(-)) controls to test for the presence of genomic DNA (gDNA) for all samples in your real-time quantitative PCR (qPCR) profiling. Just add the ValidPrime® assay to the list of assays and the gDNA control to the list of samples, and run. ValidPrime® will minimize the amount of control reactions and hence your costs as well as your efforts.


  • Offers a cost-efficient alternative to RT(-) controls
  • Accurately validates qPCR assays in terms of their sensitivity to gDNA
  • Allows correction for gDNA-derived signals
  • Reduces the need for DNase treatment

Figure 1: Total number of RT and qPCR controls needed to check for gDNA background using traditional RT(-) approach and ValidPrime®. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT(-) reactions plus m x n qPCR controls, while using ValidPrime® only m + n + 1 controls are needed.

ValidPrime® is highly optimized and specific to a non-transcribed locus of gDNA that is present in exactly one copy per haploid normal genome. Therefore, ValidPrime® measures the number of genomic copies present in a sample and can be used:

  • for normalization of samples to cell copy number
  • as endogenous control for CNV applications
  • as control for gDNA background in qPCR

For ValidPrime® Human, ValidPrime® Mouse, and ValidPrime® Vertebrate with gDNA, a gDNA standard is provided which can be used to test the sensitivity of qPCR assays for gDNA background. It is also possible to order ValidPrime® Vertebrate without gDNA.

Scientific webinar

» ValidPrime questions the need for DNase treatment in RT-qPCR experiments

Manual and product flyer

To download the manual or product flyer, please visit Product Downloads.

Following ValidPrime™ kits are available:

Species Human with gDNA
Mouse with gDNA
Vertebrate Universal - list of species in manual
with gDNA - Human, Mouse or Rat gDNA
without gDNA, combine with commercial gDNA standards
Type of assays Probe or SYBR
Kit sizes 250 rxns or 1000 rxns

Data Analysis - GenEx Enterprise

The kits also include a complimentary license for GenEx Enterprise 6 months - the easiest way to correct qPCR data for gDNA background.

1 GenEx license per customer, for first time users only.

Contacts and ordering

Place your order here in the webshop or order it by e-mailing

Shipping cost will apply, please visit General terms and Shipping for information.

Product Options
Combination of product variants is not available

Price: from €159.00

Loading Updating cart...