Control for genomic background
ValidPrime® replaces the need to perform no reverse transcriptase (RT(-)) controls to test for the presence of genomic DNA (gDNA) for all samples in your real-time quantitative PCR (qPCR) profiling. Just add the ValidPrime® assay to the list of assays and the gDNA control to the list of samples, and run. ValidPrime® will minimize the amount of control reactions and hence your costs as well as your efforts.
- Offers a cost-efficient alternative to RT(-) controls
- Accurately validates qPCR assays in terms of their sensitivity to gDNA
- Allows correction for gDNA-derived signals
- Reduces the need for DNase treatment
Figure 1: Total number of RT and qPCR controls needed to check for gDNA background using traditional RT(-) approach and ValidPrime®. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT(-) reactions plus m x n qPCR controls, while using ValidPrime® only m + n + 1 controls are needed.
ValidPrime® is highly optimized and specific to a non-transcribed locus of gDNA that is present in exactly one copy per haploid normal genome. Therefore, ValidPrime® measures the number of genomic copies present in a sample and can be used:
- for normalization of samples to cell copy number
- as endogenous control for CNV applications
- as control for gDNA background in qPCR
For ValidPrime® Human, ValidPrime® Mouse, and ValidPrime® Vertebrate with gDNA, a gDNA standard is provided which can be used to test the sensitivity of qPCR assays for gDNA background. It is also possible to order ValidPrime® Vertebrate without gDNA.
Manual and product flyer
To download the manual or product flyer, please visit Product Downloads.
Following ValidPrime™ kits are available:
|Vertebrate||Universal - list of species in manual|
|with gDNA - Human, Mouse or Rat gDNA|
|without gDNA, combine with commercial gDNA standards|
|Type of assays||Probe or SYBR|
|Kit sizes||250 rxns or 1000 rxns|
Data Analysis - GenEx Enterprise
The kits also include a complimentary license for GenEx Enterprise 6 months - the easiest way to correct qPCR data for gDNA background.
1 GenEx license per customer, for first time users only.
Contacts and ordering
Place your order here in the webshop or order it by e-mailing firstname.lastname@example.org
Shipping cost will apply, please visit General terms and Shipping for information.