Validprime™ - Control for genomic background
Minimize your qPCR costs and efforts
ValidPrime™ replaces the need to perform no reverse transcriptase (RT(-)) controls for all samples in your real-time quantitative PCR (qPCR) profiling to test for the presence of genomic DNA (gDNA). Just add the ValidPrime™ assay to the list of assays, and the gDNA control to the list of samples, and run. ValidPrime™ will minimize the amount of control reactions and hence your costs, as well as your efforts.
Table 1: Total number of RT and qPCR controls needed to check for gDNA background using traditional RT(-) approach and ValidPrime™. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT(-) reactions plus m x n qPCR controls, while using ValidPrime™ only m + n + 1 controls are needed.
ValidPrime™ is highly optimized and specific to a non-transcribed locus of gDNA that is present in exactly one copy per haploid normal genome. Therefore, ValidPrime™ measures the number of genomic copies present in a sample and can be used
- for normalization of samples to cell copy number
- as endogenous control for CNV applications
- as control for gDNA background in qPCR
For kits ValidPrime™ Human, ValidPrime™ Mouse, and ValidPrime™ Vertebrate with gDNA a gDNA standard is provided that can be used to test the sensitivity of qPCR assays for gDNA background. It is also possible to order ValidPrime™ Vertebrate without gDNA.
Following ValidPrime™ products are available:
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Species |
Human (with gDNA) |
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Type of assays |
Probe or SYBR |
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Kit sizes |
250 rxns or 1000 rxns |
Download ValidPrime flyerFor more information and how to use ValidPrime™, download the appropriate manual:
Download ValidPrime Manual - Human and Mouse - SYBR protocol Download ValidPrime Manual - Human and Mouse - Probe protocol Download ValidPrime Manual - Vertebrate - SYBR protocol Download ValidPrime Manual - Vertebrate - Probe protocol
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