Cod Uracil-DNA Glycosylase (Cod UNG) 1000 U

Eliminate problems with contaminating DNA

PCR can amplify extraordinary small amounts of RNA and DNA. The high sensitivity of these methods makes them sensitive to contaminating DNA. The contaminating DNA can be

• genomic DNA from impure RNA preps

• carryover DNA from previous PCR products

• traces of DNA from the organism used to produce the PCR enzymes (e.g. remaining E. coli DNA in your mastermix)

Prevention of contaminating DNA - use Cod UNG
Including Uracil-DNA Glycosylase (UNG) and dUTP in all PCR's help prevent carryover contamination. The Cod UNG from ArcticZymes comes from Atlantic cod, and is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. This opens up for the opportunity to do downstream applications of your PCR product, such as cloning, sequencing or genotyping using single-nucleotide primer extension technology. It is also highly advantageous when the samples have to be stored for prolonged time post-amplification. If you use Cod UNG you don't need to worry about deactivation and reactivation of your UNG enzyme, causing unwanted degradation of your PCR product.

Since Cod UNG is very efficient at low temperature and also rapidly inactivated at 50°C, UNG preincubation can be done at a temperature low enough to minimize cDNA generation during this step. These properties combined makes contamination control feasible in RT-PCR with no loss of sensitivity

• Heat-labile

• Completely and irreversibly inactivated at 50°C

• Use of Cod UNG makes contamination control possible in RT-PCR

• Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible

• High purity enzyme, tested free of contaminating nucleases

• No detectable content of E. coli DNA in enzyme product

Properties
Cod UNG hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil containing DNA leaving an apyrimidinic site in DNA. Cod UNG is not active on uracil in RNA.

Use of Cod UNG in RT-PCR
A prerequisite for using Cod UNG for contamination control in RT-PCR is that the enzyme is sufficiently heat-labile to inactivate quickly at the RT-temperature. The Cod UNG is advantageous to use in both qPCR and RT-PCR assays and is able to remove more than 10⁸ copies of contaminating DNA without affecting the sensitivity (C_q) of the assay. The fast heat inactivation and the high activity at low temperature make it possible to do the Cod UNG incubation step at room temperature for only 5 minutes prior to the amplification reaction.

For more details and information:
  Download the Cod UNG brochure

Contacts and ordering

To call for total pricing including shipping, place your order by emailing order@tataa.com