Chromofy
Price per Unit (piece): 150.00 EUR
Chromofy™
Chromofy is a monomeric asymmetric cyanine dye, developed by TATAA Biocenter for use in qPCR applications. The dye has absorbance and emission wavelengths that are suitable for the FAM/SYBR channel on most common real-time PCR instruments. When binding to dsDNA Chromofy shows a very strong fluorescence increase.
The new dye Chromofy is the by far brightest and most efficient dye for qPCR in the BIOMARK platform from Fluidigm , where it outperforms SYBRGreen, see poster 
. It is compatible with several master mixes. Chromofy is also excellent for high resolution melt (HRM) analysis.
Chromofy™ in High-resolution melting
High-resolution melting is a post-PCR analysis that discriminates different samples on the basis of their melting temperature. Chromofy and HRM can be used for genotyping, either with or without unlabelled probes and for methylation analysis. Methylation analysis is performed after bisulfite treatment of the DNA samples which converts unmethylated cytosines to uracils while methylated cytosines remain intact. During subsequent PCR the uracils will be substituted for thymines giving rise to a Tm difference between methylated and un-methylated samples, which is seen as varying heights of the plateu in the normalized HRM data. Using Chromofy your assay can be sensitive down to 1 % methylated DNA in unmethylated background.
Unit size: 25µl of 5000x solution, sufficient for over 6000 reactions.
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