Whole Genome Amplification for Single Cells or Minor Genome

9th February

TATAA Biocenter and Yikon Genomics announce strategic partnership to distribute Yikon’s MALBAC™ products for single cell profiling and YK-PGS™ Solutions in Europe

TATAA Biocenter also to introduce Yikon’s MALBAC™ in its popular NGS training courses.

> Tataa_Yikon_Press Release


 

> MALBAC™ Single Cell WGA Kit

MALBAC Single Cell WGA Kit is based on the novel patented MALBAC technology, and provides rapid, reliable and reproducible whole genome amplification from single cells or equivalent gDNA (picogram level) to produce 2-4 micrograms of amplified gDNA in about 4 hours.

The kit is able to achieve >90% amplification success rate and consistent amplification efficiency in both AT- and GC-rich regions for most cell types, such as single blastomere, polar bodies, single sperm and single cells from cultured cell lines.

The kit generates reproducible loci representation, achieving>0.9 correlation coefficient for Qpcr Ct values among reaction replicates. More than 90% of genetic loci over a genome can be amplified then analyzed by various plat forms, such as real-time PCR (qPCR) and next-generation sequencing.

The kit can also be used to amplify sub-nanogram level of inputting g DNA (e.g. from hundreds of cells).

MALBAC Single Cell WGA kit_Applications

Workflow_MALBAC single cell wga kit