Summer offers – GenMaster mix and GrandPerformance assays28th June
- Are you running a large study?
- Do you want a qPCR mastermix delivering reliable results?
- Do you want to avoid protocol optimization?
TATAA Biocenter are proud to present our new TATAA GenMaster Mixes and would like to give you a chance to try it out for an even more affordable price! During July and August you can order all sizes for the cost of an ice-cream (almost).
- Wet-lab validated probe assays
- Preamp validated to not introduce bias
- Probe assays included for free in qPCR profiling service
Highly sensitive and extensively optimized qPCR assays
TATAA GrandPerformance assays are the only assays on the market validated for single cell profiling. GrandPerformance Cancer Assays can be mixed in any combination and used in a preamplification workflow offering a sensitivity down to a few transcripts under optimum conditions. Profile individual circulating tumor cells from patients revealing heterogeneity:
*The 19% discount is valid July to August 2017 on the products in Ordering Information. Order directly from TATAA Biocenter in our webshop or by e-mail to email@example.com. The discount cannot be combined with other offers or discounts.
**The 50% discount is valid July to August 2017 on the TATAA GrandPerformance assays only. Order directly from TATAA Biocenter by e-mail to firstname.lastname@example.org. The discount cannot be combined with other offers or discounts.
Midsummer Offer – GrandMaster22nd June
Do you know where you have your primers?
Midsummer Offer – Buy GrandMaster and receive assay design and validation for 150 €!*
Original MIQE guidelines required primer disclosure, because the exact location of primers in a PCR reaction is critical. If a primer targets a sequence with variation the assay may not perform in certain samples. Many of the assays deposited in databases, published in literature and even offered commercially where developed a long time ago, when information about sequence variation was scarce.
So, even if the assays where designed according to best principles they may not perform to expectations. To check the design using updated sequence information requires knowing the primer sequence. However, most commercial assays do not disclose those and provide only a context sequence in compliance with the primer sequence disclosure added to the MIQE guidelines to satisfy industry requirements.
Further, most assays were designed for use on classical samples, where the amount of material is plenty. They perform well on those samples, but are frequently not robust and specific enough for use on more challenging samples, such as single cells and multiplex assays.
Therefore, at TATAA, we always design and validate our own assays and assays for our customers. Our validations are stringent and include estimating PCR efficiency including confidence interval, dynamic range, linearity, repeatability and Limit of Quantification. For diagnostic assays, we also include Limit of Detection including confidence interval and, when necessary, cross reactivity and robustness. Of course, all assays are validated to produce a single product and that side products such as primer dimers are negligible. One example of validation is available on Labroot.
We want you to be successful too!
In Sweden Midsummer is a major holiday, with dancing like frogs around an up-side down ancient symbol.
At TATAA we celebrate Midsummer by offering all customers that order a TATAA GrandMaster mix assay design and validation from only 150 €* through our partner!
Place your order today and you will receive an order form for the design!
*Midsummer offer valid until July 30, 2017. Design includes validation based on standard curve estimating PCR efficiency incl., confidence interval, dynamic range, R2, and LoQ. Melt curve analysis is performed to validate a single product. NTC is run to validate negligible primer dimer formation. The design and validation is done by our partner GeneCore at BIOCEV.
Expression profiling of single circulating tumor cells16th June
TATAA Biocenter launches GrandPerformance assays for expression profiling of single circulating tumor cells
TATAA Biocenter, Europe´s leading provider of genomic services using quantitative real-time PCR (qPCR), launches the GrandPerformance qPCR assays for the profiling of Circulating Tumor Cells. The assays are optimized for single cell sensitivity and the workflow is based on preamplification allowing sensitive and precise measurements of 96 specific expression markers per cell.
Tumors may shed cells into the blood flow that evade the immune defence and may seed metastases. These cells are called circulating tumor cells (CTCs). CTCs may be detected in blood sample and their presence is a negative prognostic indicator. Further characterization of CTCs by expression profiling may reveal their phenotype, which may guide therapeutic decisions. Molecular characterization of CTCs is challenging, because of their very low abundance, which requires exceedingly sensitive and specific methods. TATAA Biocenter has previously developed workflow for exceedingly specific and sensitive single cell expression profiling based on reverse transcription (RT), preamplification and real-time PCR (Expert review of molecular diagnostics 14, 323 (2014)). Now TATAA launches a panel of over 100 GrandPerformance assays wet-lab validated for CTC profiling that allows the characterization of individual CTCs. The panel was recently used by the group of professor Klaus Pantel at University Klinik Hamburg-Eppendorf to profile individual CTCs collected from patient samples revealing heterogeneity among them (Clinical Chemistry 62 (11), 1504-1515 (2016)).
About TATAA Biocenter
TATAA Biocenter is the world’s largest organizer of hands-on training in quantitative real-time PCR and Next Generation Sequencing, Europe’s leading provider of nucleic acid analysis services, and Sweden’s most comprehensive distributor of products for nucleic acids analysis. TATAA Biocenter offers a seamless workflow for high quality nucleic acids analysis from experimental design, sample preparation, quality control, and analysis, meeting the specific requirements for compliance with ISO17025 standard. Founded in 2001, TATAA Biocenter has laboratories in Gothenburg, Sweden, and in Prague, Czech Republic. In 2013 TATAA Biocenter was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material.
For more information on the TATAA GrandPerformance assays, see https://webshop.tataa.com/category.html/qpcr-assays or contact us on email@example.com