LGC researchers use ValidPrime™
2nd JuneLGC researchers use ValidPrime™ for Standardization of cell-free DNA measurement in plasma, controlling for extraction efficiency, fragment size bias and quantification.
» Go to article “Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification“
Figure: Total number of RT and qPCR controls needed to check for gDNA background using traditional RT(-) approach and ValidPrime™. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT(-) reactions plus m x n qPCR controls, while using ValidPrime™ only m + n + 1 controls are needed.
