Protein profiling

High-throughput sensitive protein quantification using qPCR-coupled immunoassays

TATAA Biocenter offers to perform your high-throughput protein biomarker discovery using qPCR and the latest technology. Our experts pioneered qPCR based nucleic acid analyses in Europe and have more than a decade of experience of projects and research within the field.

» TATAA Biocenter is a certified service provider by Olink Proteomics

Precision proteomics for biomarker discovery

Biomarker studies which were previously limited by poor assay sensitivity, low throughput systems and insufficient sample amount are now feasible in a simple and efficient way.

For the first time it is possible to do high capacity quantification of 92 protein biomarkers in 96 samples. Conventional immunoassays have been unable to scale due to increasing antibody cross-reactivity but TATAA Biocenter offers a solution using the Multiplex platform from Olink Proteomics. It generates high quality data even in highly multiplexed formats and the opportunities are enormous for powerful analysis of multivariate biomarker patterns.

Figure 1. BioMark™ 96×96 chip generates
9,216 data points in a single run

The quantification is performed using the Fluidigm BioMark™ HD Real-time PCR Platform and generates 9,216 data points in a single run (Figure 1.).

  • Discover novel expression patterns of 92 protein biomarkers in precious biological materials.
  • High-multiplex analysis with no compromise on data quality.

TATAA uses a fast and sensitive method based on the Proximity Extension Assay (PEA®) technology. The method allows for rapid and easy testing of potential biomarkers and each assay in the 92 biomarker panel has been accurately validated for sensitivity, recovery, dynamic range, specificity and precision.

Unravel low-abundant proteins not detected before

The sensitivity of qPCR allows extremely low sample consumption. TATAA can generate more information from less sample volume which enables you to make the most out of your precious biobanked samples.

  • Down to sub pg/ml sensitivity in only 1 µl samples

Sample types

  • Plasma
  • Serum
  • Tissue
  • And more, contact us for further information

Services offered

Protein expression analysis and pathway analysis with:

Validation data

The analytical performance of the panels is carefully validated for sensitivity, recovery, dynamic range, specificity, precision and scalability. You can see the performance and report on this validation by accessing download links for the complete Data Validation Document for each panel.

Publication list

To see how scientists around the world have been successfully using Proseek panels to advance their biomarker research, take a look at the latest publication list.

Contact

For other projects of your choice, or the mentioned above, please feel free to contact us for requests and more information .

Proseek is a trademark of Olink AB. Proseek® Multiplex is for research use only. Not for use in human diagnostics or therapeutic procedures. Proseek® Multiplex is sold under license from PHRI Properties, Inc., and may be used under the PHRI Properties patent rights outside the field of human in vitro diagnostics.