Single cell profiling study published

10th November

Publication –  single cell profiling study of human adult germ stem cells

TATAA researchers in collaboration with Thomas Skutellas group at Heidelberg University.

>> Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells

CelluLyser™ Micro Lysis and cDNA Synthesis Kit – 1-500 cells

> TATAA PreAmp GrandMaster® Mix

> TATAA GrandPerformance assays

The aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen−/laminin+ binding-) selected fraction of enriched spermatogonia. Single-cell transcriptional profiling by Fluidigm BioMark system of a long-term cultured haGSCs cluster in comparison to human embryonic stem cells (hESCs) and human fibroblasts (hFibs) revealed that haGSCs showed a characteristic germ- and pluripotency-associated gene expression profile with some similarities to hESCs and with a significant distinction from somatic hFibs. Genome-wide comparisons with microarray analysis confirmed that different haGSC colonies exhibited gene expression heterogeneity with more or less pluripotency.The results of this study confirm that haGSCs are adult stem cells with a specific molecular gene expression profile in vitro, related but not identical to true pluripotent stem cells. Under ES-cell conditions haGSC colonies could be selected andmaintained in a partial pluripotent state at themolecular level, whichmay be related to their cell plasticity and potential to differentiate into cells of all germ layers.